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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBX1 Monoclonal antibody

RBX1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F4D9

Cat no : 66716-1-Ig

Synonyms

BA554C12.1, Protein ZYP, RBX1, Regulator of cullins 1, ring box 1, RING box protein 1, RING finger protein 75, RNF75, ROC1

Validation Data Gallery

Applications:
  • WB analysis using 66716-1-Ig

    WB analysis using 66716-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66716-1-Ig (RBX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of RAW 264.7 using 66716-1-Ig

    WB analysis of RAW 264.7 using 66716-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66716-1-Ig (RBX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66716-1-Ig

    IHC staining of human liver cancer using 66716-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66716-1-Ig (RBX1 antibody) at dilution of 1:5000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with RBX1 antibody included
  • IHC staining of human liver cancer using 66716-1-Ig

    IHC staining of human liver cancer using 66716-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66716-1-Ig (RBX1 antibody) at dilution of 1:5000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with RBX1 antibody included

Tested Applications

Positive WB detected inHeLa cells, RAW 264.7 cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RBX1 Ready-To-Use IHC Kit. RBX1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Sample-dependent, check data in validation data gallery

Product Information

66716-1-Ig targets RBX1 in WB, IP, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RBX1 fusion protein Ag7005 相同性解析による交差性が予測される生物種
Full Name ring-box 1
Calculated molecular weight 12 kDa
Observed molecular weight 12-15 kDa
GenBank accession numberBC001466
Gene symbol RBX1
Gene ID (NCBI) 9978
RRIDAB_2882067
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40. It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.

Protocols

Product Specific Protocols
WB protocol for RBX1 antibody 66716-1-IgDownload protocol
IHC protocol for RBX1 antibody 66716-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

bioRxiv

The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation

Authors - Magda Wąchalska
View Article