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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RASA2/3 Polyclonal antibody

RASA2/3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27835-1-AP

Synonyms

GAP1(IP4BP), GAP1IP4BP, GAPIII, Ins P4 binding protein, RAS p21 protein activator 3, RASA2/3, RASA3

Validation Data Gallery

Applications:
  • WB analysis of 3T3-L1 using 27835-1-AP

    WB analysis of 3T3-L1 using 27835-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 27835-1-AP (RASA2/3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 27835-1-AP

    WB analysis of HeLa using 27835-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27835-1-AP (RASA2/3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected in3T3-L1 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

27835-1-AP targets RASA2/3 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RASA2/3 fusion protein Ag27152 相同性解析による交差性が予測される生物種
Full Name RAS p21 protein activator 3
Calculated molecular weight 834 aa, 96 kDa
Observed molecular weight 96 kDa
GenBank accession numberBC038456
Gene symbol RASA3
Gene ID (NCBI) 22821
RRIDAB_2880988
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rasa3 is a GTPase activating protein of the GAP1 family which targets R-Ras and Rap1. While R-Ras has been extensively studied due to its involvement in cancer, Rap1 has recently attracted a lot of attention due to its central role in development and morphogenesis of higher organisms, especially in the cardiovasculature. This antibody can react with RASA2 and RASA3.

Protocols

Product Specific Protocols
WB protocol for RASA2/3 antibody 27835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Arterioscler Thromb Vasc Biol

Lipid Receptor GPR31 (G-Protein-Coupled Receptor 31) Regulates Platelet Reactivity and Thrombosis Without Affecting Hemostasis.

Authors - Layla Van Doren
View Article
mouseWB

Front Immunol

The Rac-GEF Tiam1 controls integrin-dependent neutrophil responses

Authors - Kirsti Hornigold
View Article