PSPC1 Polyclonal antibody

PSPC1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 16714-1-AP

Synonyms

paraspeckle component 1, Paraspeckle protein 1, PSP1, PSPC1



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, PC-3 cells
Positive IP detected inHEK-293 cells
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16714-1-AP targets PSPC1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSPC1 fusion protein Ag10136 相同性解析による交差性が予測される生物種
Full Name paraspeckle component 1
Calculated molecular weight 523 aa, 59 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC014184
Gene symbol PSPC1
Gene ID (NCBI) 55269
RRIDAB_2878302
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSPC1 (paraspeckle component 1), a known paraspeckle biomarker, is a putative transcription factor that belongs to the Drosophila behavior/human splicing (DBHS) family. PSPC1 was recently identified as a contextual determinant of tumor progression in multiple cancer types involving oncogenic reprogramming to switch proapoptotic TGF-β to prometastatic TGF-β via hijacking of Smad2/3 targeting. Upregulated PSPC1 is correlated with advanced tumor stages and poor survival of patients with breast, lung, and liver cancers. Upregulated PSPC1 potentiates expression of mesenchymal markers, EMT transcription factors (EMT-TF), cancer stem-like cell transcription factors (CSC-TF), and c-Myc-related proliferation genes, thus promoting migration, invasion, spheroid formation, tumor formation, and metastasis.

Protocols

Product Specific Protocols
WB protocol for PSPC1 antibody 16714-1-APDownload protocol
IF protocol for PSPC1 antibody 16714-1-APDownload protocol
IP protocol for PSPC1 antibody 16714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Drug Resist Updat

Cell membrane-camouflaged bufalin targets NOD2 and overcomes multidrug resistance in pancreatic cancer

Authors - Wei Zhang
humanWB

Nat Commun

K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis

Authors - Xiao-Lan Zhang
humanWB

Nucleic Acids Res

mRNAs are sorted for export or degradation before passing through nuclear speckles.

Authors - Jing Fan
mouseWB

Int J Mol Sci

The KDET Motif in the Intracellular Domain of the Cell Adhesion Molecule L1 Interacts with Several Nuclear, Cytoplasmic, and Mitochondrial Proteins Essential for Neuronal Functions

Authors - Ralf Kleene
  • KD Validated
mouseIF

Int J Mol Sci

The Interactions of the 70 kDa Fragment of Cell Adhesion Molecule L1 with Topoisomerase 1, Peroxisome Proliferator-Activated Receptor γ and NADH Dehydrogenase (Ubiquinone) Flavoprotein 2 Are Involved in Gene Expression and Neuronal L1-Dependent Functions

Authors - Gabriele Loers
humanWB

J Biol Chem

The disordered C terminus of ALKBH5 promotes phase separation and paraspeckles assembly

Authors - Xiaoyang Qin