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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NDUFB4 Polyclonal antibody

NDUFB4 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 27931-1-AP

Synonyms

B15, CI B15, Complex I B15, NDUFB4

Validation Data Gallery

Applications:
  • WB analysis of mouse heart using 27931-1-AP

    WB analysis of mouse heart using 27931-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 27931-1-AP (NDUFB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 27931-1-AP

    IF Staining of HeLa using 27931-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 27931-1-AP (NDUFB4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse heart tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27931-1-AP targets NDUFB4 in WB, IF,ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFB4 fusion protein Ag27484 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa
Calculated molecular weight 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC000855
Gene symbol NDUFB4
Gene ID (NCBI) 4710
RRIDAB_2881012
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NDUFB4 antibody 27931-1-APDownload protocol
IF protocol for NDUFB4 antibody 27931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols