Mouse MIF ELISA Kit0 Publications

カタログ番号: KE10027

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse MIF ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
2.3 pg/mL

測定範囲:
31.25 - 2000 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatants 109% 86%-119%
Mouse serum 104% 87%-117%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 47.1 2.9 6.2
2 20 423.9 16.5 3.8
3 20 1,663.7 99.9 6.0

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 40.6 2.7 6.5
2 24 391.2 25.0 6.3
3 24 1,645.0 107.9 6.5

製品概要
KE10027 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MIF ELISA kit is to be used to detect and quantify protein levels of endogenous MIF. The assay recognizes mouse MIF. A polyclonal antibody specific for MIF has been pre-coated onto the microwells. The MIF protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for mouse MIF is added to detect the captured mouse MIF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
GIF, Glif, Mif
背景説明

Macrophage migration inhibitory factor (MIF) is an upstream regulator of the innate and adaptive immune response that is widely expressed and that exhibits chemokine-like activities. MIF play a role in the pathogenesis of inflammatory diseases, such as atherosclerosis, rheumatoid arthritis, sepsis, asthma, and acute respiratory distress syndrome. MIF is expressed by the ischemic myocardium and that it may exhibit an overall cardioprotective role during I/R injury. MIF regulates inflammatory processes by recruiting immune cells, amplifying cytokine production, inhibiting apoptosis and counter-regulating the immunosuppressive action of glucocorticoids.


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