Mouse IL5 ELISA Kit 0 Publications

カタログ番号: KE10018

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse IL5 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
1.0 pg/mL

測定範囲:
7.8-500 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 94% 82%-108%
Mouse serum 90% 76%-116%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 10.3 0.4 3.9
2 20 108.0 2.0 1.9
3 20 556.4 21.6 4.7

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 10.4 0.5 4.9
2 24 111.4 1.8 1.6
3 24 503.0 17.4 3.5

製品概要
KE10018 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL5 ELISA kit is to be used to detect and quantify protein levels of endogenous IL5. The assay recognizes mouse IL5. A monoclonal antibody specific for IL5 has been pre-coated onto the microwells. The IL5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for IL5 is added to detect the captured IL5 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
B-cell growth factor II, BCGF-II, Il 5, Il5, IL-5, interleukin 5, Interleukin-5
背景説明

Interleukin-5, or IL-5, was originally discovered as a soluble T cell-derived factor, called T cell-replacing factor (TRF), that induced T cell-depleted activated B cells to secrete immunoglobulin. IL-5 is a key hematopoietic cytokine in eosinophil differentiation, maturation, recruitment and activation at sites of allergic inflammation. IL-5 also plays a role in the development, metabolism, and function of basophils. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of at least two chains: an α chain that binds IL-5 with low affinity and a βchain that does not bind IL-5, but together with the IL-5α chain, constitutes the high affinity IL-5 receptor. The β chain is common to the IL-3, IL-5 and Gm-csf receptors and has been shown to signal through the JAK/Stat pathway.


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