Mouse IL27 ELISA Kit0 Publications

カタログ番号: KE10016

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse IL27 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
2.0 pg/mL

測定範囲:
31.25 - 2000 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 97% 87%-109%
Mouse serum 74% 70%-80%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 440.7 21.4 4.9
2 20 622.6 35.7 5.7
3 20 1,271.1 84.5 6.6

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 346.9 31.6 9.1
2 24 766.0 56.6 7.4
3 24 1,491.3 140.3 9.4

製品概要
KE10016 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL27 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL27. The assay recognizes mouse IL27. A monoclonal antibody specific for mouse IL27 has been pre-coated onto the microwells. The mouse IL27 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for mouse IL27 is added to detect the captured mouse IL27 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
IL 27, IL 27p28, Il27, IL-27, Il30, interleukin 27, Interleukin-27, p28
背景説明

IL27, a member of IL12/IL23 heterodimeric family of cytokines, has pleiotropic properties that can enhance or limit immune responses. IL27 is a heterodimeric cytokine composed of two subunits: IL27a and IL27b. IL27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL10 producing type 1 regulatory T cells.


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