Mouse IL17A ELISA Kit 0 Publications

カタログ番号: KE10020

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse IL17A ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
2.6 pg/mL

測定範囲:
7.8-500 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 93% 87%-105%
Mouse serum 93% 74%-112%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 9.9 0.8 8.0
2 20 104.5 4.5 4.3
3 20 386.5 26.1 6.8

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 10.2 1.0 9.6
2 24 93.7 8.6 9.2
3 24 428.2 42.1 9.8

製品概要
KE10020 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL17A ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL17A. The assay recognizes mouse IL17A. A monoclonal antibody specific for mouse IL17A has been pre-coated onto the microwells. The mouse IL17A protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for mouse IL17A is added to detect the captured mouse IL17A protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Ctla 8, Ctla8, CTLA-8, Il17, IL-17, Il17a, IL-17A, interleukin 17A, Interleukin-17A
背景説明

Il17a, also named as Il-17, is a proinflammatory cytokine. Il-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The Il-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. Il-17 and IL-22, are highly increased.


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