Mouse IL15 ELISA Kit0 Publications

カタログ番号: KE10028

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse IL15 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum,Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
50.2 pg/mL

測定範囲:
250 -8000 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Mouse serum 91% 81%-97%
Cell culture supernatants 110% 99%-124%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 323.3 23.3 7.2
2 20 837.1 52.1 6.2
3 20 3,300.7 215.3 6.5

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 242.7 21.0 8.6
2 24 868.7 68.0 7.8
3 24 3,355.6 201.9 6.0

製品概要
KE10028 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL15 ELISA kit is to be used to detect and quantify protein levels of endogenous IL15. The assay recognizes mouse IL15. A monoclonal antibody specific for IL15 has been pre-coated onto the microwells. The IL15 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for IL15 is added to detect the captured IL15 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
AI503618, Il15, IL-15, interleukin 15
背景説明

Interleukin 15 (Il15) is a cytokine important for the development, maturation, and function of many cells of the immune system including NK, NKT, gammadeltaT, and CD8(+) T cells. Its expression affects homeostasis and function of B cells through NK cell-derived IFNG. Il-15 binds a trimeric Il-15 receptor (Il-15R) complex formed by a specific receptor chain (Il-15Rα) combined with theβandγchains of the IL-2R, acting as signal transducing components. The transgenic mice overexpressing Il15 exhibit an increase in the number of memory CD8+ T cells in a naive state and enhanced protection against bacterial infections. Mice lacking Il15 exhibit impaired protection against a strain of attenuated Mycobacterium.


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