Mouse IL13 ELISA Kit0 Publications

カタログ番号: KE10021

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse IL13 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Cell culture supernatant

アッセイ方法:
Sandwich

感度:
1.5 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 98% 86%-111%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 20.9 2.0 9.7
2 20 223.1 14.1 6.3
3 20 942.7 55.5 5.9

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 63.6 5.1 8.0
2 24 264.1 12.5 4.7
3 24 868.6 45.6 5.3

製品概要
KE10021 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL13 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL13. The assay recognizes mouse IL13. A monoclonal antibody specific for mouse IL13 has been pre-coated onto the microwells. The mouse IL13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for mouse IL13 is added to detect the captured mouse IL13 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Il 13, Il13, IL-13, interleukin 13, Interleukin-13, T-cell activation protein P600
背景説明

Interleukin 13 (IL-13) is an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. IL-13 up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. IL-13 inhibits the production of a series of cytokines like IL-1, IL-6, TNF-alpha, and IL-8 by activated human monocytes. IL-13 induces IFN-gamma production by NK cells. IL-13 is thought to be important cytokine in the pathogenesis of asthma, and more recently has been shown to play a pivotal role in a number of fibrotic diseases including hepatic and pulmonary fibrosis, and nodular sclerosing Hodgkin’s disease.


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