Mouse G-CSF ELISA Kit0 Publications

カタログ番号: KE10025

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse G-CSF ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
0.8 pg/mL

測定範囲:
7.8-500 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Mouse serum 95% 74%-127%
Cell culture supernatants 105% 85%-124%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 216.2 7.1 3.3
2 20 85.3 2.5 2.9
3 20 8.8 0.9 9.7

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 209.1 10.2 4.9
2 24 86.2 4.6 5.3
3 24 51.2 3.8 7.4

製品概要
KE10025 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse G-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous mouse G-CSF. The assay recognizes mouse G-CSF. A monoclonal antibody specific for mouse G-CSF has been pre-coated onto the microwells. The mouse G-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for mouse G-CSF is added to detect the captured mouse G-CSF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Csf3, Csfg, G CSF, G-CSF, MGI IG
背景説明

Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT) and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.


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