Mouse CXCL2 ELISA Kit0 Publications

カタログ番号: KE10022

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse CXCL2 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
20.5 pg/mL

測定範囲:
31.25 - 500 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 100% 82%-121%
Mouse serum 86% 72%-133%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 438.8 13.6 3.1
2 20 137.6 4.4 3.2
3 20 30.7 1.6 5.1

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 443.1 12.1 2.7
2 24 145.2 4.4 3.0
3 24 30.0 1.5 5.1

製品概要
KE10022 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CXCL2 ELISA kit is to be used to detect and quantify protein levels of endogenous CXCL2. The assay recognizes mouse CXCL2. A monoclonal antibody specific for CXCL2 has been pre-coated onto the microwells. The CXCL2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for CXCL2 is added to detect the captured CXCL2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
CINC 2a, C-X-C motif chemokine 2, Cxcl2, Gro2, GROb, GRO-beta-T, Mgsa b, MIP 2, MIP 2a, Mip2, MIP2-alpha, Scyb, Scyb2
背景説明

Cxcl2, also known as macrophage inflammatory protein 2-alpha (Mip2-alpha), is a member of CXC chemokine family that binds to CXC chemokine receptor 2 (Cxcr2). Cxcl2 is mainly produced by macrophages, endothelial cells, epithelial cells and tumor cells. Cxcl2 plays important roles in various biological progresses such as angiogenesis, inflammation, immune response and cancer biological behaviors. Under inflammatory conditions in CNS, microglia and astrocytes may secrete Cxcl2 as well as other chemokines, which induces chemotaxis and infiltration of circulatory neutrophils. Indeed, previous studies suggest that Cxcl2 is involved in Alzheimer disease, multiple sclerosis and brain ischemia.


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