Mouse CXCL1 ELISA Kit 0 Publications

カタログ番号: KE10019

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse CXCL1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
6.5 pg/mL

測定範囲:
31.25-1000 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 99% 72%-121%
Mouse serum 90% 71%-118%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 30.1 1.0 3.3
2 20 120.7 3.5 2.9
3 20 481.3 18.0 3.7

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 31.8 1.7 5.2
2 24 130.1 4.9 3.8
3 24 505.0 14.6 2.9

製品概要
KE10019 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CXCL1 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CXCL1. The assay recognizes mouse CXCL1. A monoclonal antibody specific for mouse CXCL1 has been pre-coated onto the microwells. The mouse CXCL1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for mouse CXCL1 is added to detect the captured mouse CXCL1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
C-X-C motif chemokine 1, Cxcl1, Fsp, gro, Gro1, GRO-alpha, KC, Mgsa, N51, NAP-3, Scyb1
背景説明

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.


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