Mouse CCL3 ELISA Kit0 Publications

カタログ番号: KE10023

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse CCL3 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
0.03 pg/mL

測定範囲:
1.95 - 125 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Mouse serum 107% 75%-126%
Cell culture supernatants 104% 87%-123%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 86.8 3.8 4.4
2 20 25.8 0.9 3.5
3 20 5.3 0.1 2.5

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 83.8 3.9 4.7
2 24 25.7 0.8 3.0
3 24 5.1 0.3 5.4

製品概要
KE10023 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse CCL3 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CCL3. The assay recognizes mouse CCL3. A polyclonal antibody specific for mouse CCL3 has been pre-coated onto the microwells. The mouse CCL3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for mouse CCL3 is added to detect the captured mouse CCL3 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
AI323804, Ccl3, G0S19 1, LD78alpha, LD78-alpha, MIP 1alpha, MIP1 (a), MIP1 alpha, Mip1a, MIP-1-alpha, Scya3, SIS-beta
背景説明

Chemokine (C-C motif) ligand 3 (CCL3), also known as MIP-1 alpha, belongs to the family of chemokines. CCL3 has been found in the central nervous system and its cognate receptors, CCR1 and CCR5, have been reported to be expressed by astrocytes, microglia and neurons. CCL3 and its receptors, CCR1 and CCR5, also contribute to the development of bone disease in multiple myeloma by supporting tumor growth and regulating osteoclast differentiation. CCL3 is also associated with the regulation of cell growth, angiogenesis, and metastasis of different tumors such as melanoma, renal cell carcinoma, and colorectal cancer. Moreover, CCL3 enhances cell migration and metastasis by up-regulating matrix metalloproteinase-2 (MMP)-2 expression in chondrosarcoma cells.


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