Mouse BAFF ELISA Kit0 Publications

カタログ番号: KE10026

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

販売および在庫状況はコスモ・バイオ(株)にお問い合わせください。
distributor

Images See all

概要


製品名:
Mouse BAFF ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plamsa, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
23.0 pg/mL

測定範囲:
62.5-4000 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Mouse serum 100% 71%-122%
Cell culture supernatants 93% 79%-104%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 1,662.4 52.3 3.1
2 20 1,980.2 69.9 3.5
3 20 3,106.9 109.0 3.5

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 1,644.7 68.4 4.2
2 24 1,890.0 79.8 4.2
3 24 2,636.9 258.8 9.8

製品概要
KE10026 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The BAFF ELISA kit is to be used to detect and quantify protein levels of endogenous BAFF. The assay recognizes mouse BAFF. A monoclonal antibody specific for BAFF has been pre-coated onto the microwells. The BAFF protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for BAFF is added to detect the captured BAFF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
BAFF, BLyS, D8Ertd387e, TALL 1, TALL1, THANK, Tnfsf13b, TNFSF20, zTNF4
背景説明

TNFSF13B, also named as BAFF, BLYS, TALL1, TNFSF20, ZTNF4 and CD257, is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13B is localized in chromosome region 13q32-q34 and is implicated in the regulation of immune response to infections, autoimmune disease, and inflammation. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. At the placental level, it is believed that TNFSF13B has an antiapoptotic effect. TNFSF13B is overexpressed in several autoimmune patients although the mechanism behind this feature is unclear.


Back
to top