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  • Featured Product
  • KD/KO Validated

MRPL45 Monoclonal antibody

MRPL45 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B4B4

Cat no : 66531-1-Ig

Synonyms

L45mt, MRPL45, MRP-L45

Validation Data Gallery

Applications:
  • WB analysis using 66531-1-Ig

    WB analysis using 66531-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66531-1-Ig

    WB analysis of HeLa using 66531-1-Ig

    WB result of MRPL45 antibody (66531-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL45 transfected HeLa cells.

  • WB analysis of RAW 264.7 using 66531-1-Ig

    WB analysis of RAW 264.7 using 66531-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 66531-1-Ig

    WB analysis of HSC-T6 using 66531-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66531-1-Ig

    IHC staining of human breast cancer using 66531-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 66531-1-Ig

    IHC staining of human breast cancer using 66531-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, RAW 264.7 cells, HEK-293 cells, Jurkat cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

66531-1-Ig targets MRPL45 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MRPL45 fusion protein Ag8207 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L45
Calculated molecular weight 35 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC006235
Gene symbol MRPL45
Gene ID (NCBI) 84311
RRIDAB_2881894
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL45 is a 39S subunit protein.

Protocols

Product Specific Protocols
WB protocol for MRPL45 antibody 66531-1-IgDownload protocol
IHC protocol for MRPL45 antibody 66531-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols