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  • KD/KO Validated

LTBR Polyclonal antibody

LTBR Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20331-1-AP

Synonyms

D12S370, LT BETA R, LTBR, Lymphotoxin beta receptor, TNF R III, TNF RIII, TNFCR, TNFR III, TNFR RP, TNFR2 RP, TNFR3, TNFRSF3



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20331-1-AP is LTBR Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LTBR fusion protein Ag14065 相同性解析による交差性が予測される生物種
Full Name lymphotoxin beta receptor (TNFR superfamily, member 3)
Calculated molecular weight 435 aa, 47 kDa
Observed molecular weight 47-60 kDa
GenBank accession numberBC026262
Gene symbol LTBR
Gene ID (NCBI) 4055
RRIDAB_10694276
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LTBR antibody 20331-1-APDownload protocol
IHC protocol for LTBR antibody 20331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Mol Ther

Activation of NF-κB-Inducing Kinase in Islet β Cells Causes β Cell Failure and Diabetes.

Authors - Xinzhi Li
humanWB

J Immunol

SNX17 Affects T Cell Activation by Regulating TCR and Integrin Recycling.

Authors - Douglas G Osborne
humanIHC,IF,WB

Am J Pathol

Lymphotoxins Promote the Progression of Human Lymphatic Malformation by Enhancing Lymphatic Endothelial Cell Proliferation.

Authors - Jie-Gang Yang
  • KD Validated

Mol Med Rep

Lymphotoxin β receptor activation promotes mRNA expression of RelA and pro-inflammatory cytokines TNFα and IL-1β in bladder cancer cells.

Authors - Mo Shen
mouseIF

Theranostics

Islet α-cell Inflammation Induced By NF-κB inducing kinase (NIK) Leads to Hypoglycemia, Pancreatitis, Growth Retardation, and Postnatal Death in Mice.

Authors - Xinzhi Li
humanIHC,IF

J Reprod Immunol

A LIGHT-HVEM/LTβR axis contributes to the fibrosis of intrauterine adhesion

Authors - Ayitila Abudukeyoumu