CD11c/Integrin Alpha X Polyclonal antibody
CD11c/Integrin Alpha X Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 17342-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human blood tissue |
| Positive IHC detected in | human tonsillitis tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HL-60 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 11 publications below |
| IHC | See 22 publications below |
| IF | See 15 publications below |
| FC | See 4 publications below |
Product Information
17342-1-AP targets CD11c/Integrin Alpha X in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD11c/Integrin Alpha X fusion protein Ag11380 相同性解析による交差性が予測される生物種 |
| Full Name | integrin, alpha X (complement component 3 receptor 4 subunit) |
| Calculated molecular weight | 1169 aa, 129 kDa |
| Observed molecular weight | 150 kDa |
| GenBank accession number | BC038237 |
| Gene symbol | ITGAX |
| Gene ID (NCBI) | 3687 |
| RRID | AB_2129787 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD11c/Integrin Alpha X antibody 17342-1-AP | Download protocol |
| IHC protocol for CD11c/Integrin Alpha X antibody 17342-1-AP | Download protocol |
| FC protocol for CD11c/Integrin Alpha X antibody 17342-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Acta Pharm Sin B Celastrol targets adenylyl cyclase-associated protein 1 to reduce macrophages-mediated inflammation and ameliorates high fat diet-induced metabolic syndrome in mice. | ||
Biomaterials Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia. | ||
Bioact Mater Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect. | ||
Theranostics Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis. |
