Human PGⅡ ELISA Kit0 Publications

カタログ番号: KE00090

93,000円

(希望販売価格)

容量: 1 Kit (96 assays)

販売および在庫状況はコスモ・バイオ(株)にお問い合わせください。
distributor

Images See all

概要


製品名:
Human PGⅡ ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
0.03 ng/mL

測定範囲:
1.56-100 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 92% 86%-101%
Human plasma 99% 75%-128%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 5.4 0.2 4.3
2 20 25.2 0.9 3.4
3 20 89.9 6.0 6.7

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 5.6 0.2 3.1
2 24 24.4 0.6 2.4
3 24 80.8 4.9 6.1

製品概要
KE00090 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PGⅡ ELISA kit is to be used to detect and quantify protein levels of endogenous PGⅡ. The assay recognizes human PGⅡ. A polyclonal antibody specific for PGⅡ has been pre-coated onto the microwells. The PGⅡ protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for PGⅡ is added to detect the captured PGⅡprotein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Gastricsin, Pepsinogen C, PEPSINOGEN, GROUP II, PGC, Progastricsin, progastricsin (pepsinogen C)
背景説明

PepsinogenⅠand Ⅱ, as precursors of pepsin, are produced by gastric mucosa and released into the gastric lumen and peripheral circulation. The advanced inflammation of gastric mucosa and its progression toward atrophic gastritis (AG) in the corpus is associated with a change in serum biomarkers pepsinogenⅠand Ⅱ.(PMID:23496362). PepsinogenⅡ also known as progastricsin or PGC (pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis.


Back
to top