Human Uteroglobin ELISA Kit0 Publications

カタログ番号: KE00099

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human Uteroglobin ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant, Urine

アッセイ方法:
Sandwich

感度:
0.03 ng/mL

測定範囲:
0.156 - 10 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 100% 80%-120%
Urine 99% 83%-119%
Human plasma 100% 78%-121%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 0.2 0.01 5.4
2 20 2.5 0.18 7.4
3 20 9.8 0.74 7.6

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 0.2 0.02 9.4
2 24 2.6 0.23 7.4
3 24 10.8 1.00 9.2

製品概要
KE00099 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Uteroglobin ELISA kit is to be used to detect and quantify protein levels of endogenous Uteroglobin. The assay recognizes human Uteroglobin. A polyclonal antibody specific for Uteroglobin has been pre-coated onto the microwells. The Uteroglobin protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for Uteroglobin is added to detect the captured Uteroglobin protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
CC10, CC16, CCPBP, CCSP, SCGB1A1, UGB, UP 1, UP1, Urinary protein 1, Urine protein 1, Uteroglobin
背景説明

Uteroglobin, also named clara cell secretory protein or CC16, is mainly secreted by the club cells along the tracheobronchial tree, diffuses passively into the bloodstream and is rapidly eliminated by glomerular filtration. It is a steroid-dependent, immunomodulatory, cytokine-like protein, and it is secreted by the mucosal epithelial cells of many vertebrates and takes part in some signaling pathways. Growing evidence indicates that Uteroglobin has anti-inflammatory and anti-toxicant properties in the lung and may protect against obstructive lung diseases. lower levels of Uteroglobin in blood and airways have been associated with prevalence and severity of Chronic obstructive pulmonary disease (COPD). Defects in Uteroglobin are associated with a susceptibility to asthma.


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