Human TNFR1 ELISA Kit0 Publications

カタログ番号: KE00051

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human TNFR1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Urine

アッセイ方法:
Sandwich

感度:
0.1 pg/mL

測定範囲:
3.9-250 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Urine 94% 83%-110%
Human plasma 91% 75%-104%
Human serum 94% 86%-109%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 128.5 2.30 1.8
2 20 31.7 0.67 2.1
3 20 7.4 0.28 3.8

InterAssay:
Samplenmean (pg/mL)SDCV%
1 20 141.2 11.01 7.8
2 20 33.0 2.38 7.2
3 20 5.4 0.48 8.9

製品概要
KE00051 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNFR1 ELISA kit is to be used to detect and quantify protein levels of endogenous TNFR1. The assay recognizes human TNFR1. A monoclonal antibody specific for TNFR1 has been pre-coated onto the microwells. The TNFR1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for TNFR1 is added to detect the captured TNFR1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm .

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
CD120a, FPF, p55, p55 R, p60, TBP1, TBPI, TNF R, TNF R I, TNF R1, TNF R55, TNF RI, TNFAR, TNFR I, TNFR1, TNFR55, TNFR60, TNFRSF1A
背景説明

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling. This receptor can be released to the extracellular space by two mechanisms, ectodomain shedding and constitutive release of full-length 55-kDa TNFR1. Soluble TNFR1 could function as TNF-binding protein, inhibiting TNF bioactivity.


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