Human ST2 ELISA Kit0 Publications

カタログ番号: KE00055

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human ST2 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
1.8 pg/mL

測定範囲:
31.25-2000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 93% 78%-107%
Human plasma 91% 82%-103%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 1,058.5 30.8 2.9
2 20 247.2 5.6 2.3
3 20 61.9 1.6 2.6

InterAssay:
Samplenmean (pg/mL)SDCV%
1 20 1,054.8 40.8 3.9
2 20 257.3 7.1 2.7
3 20 72.4 4.4 6.1

製品概要
KE00055 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ST2 ELISA kit is to be used to detect and quantify protein levels of endogenous ST2. The assay recognizes human ST2. A monoclonal antibody specific for ST2 has been pre-coated onto the microwells. The ST2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for ST2 is added to detect the captured ST2 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
DER4, FIT 1, IL1RL1, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1
背景説明

Protein ST2, also known as IL1RL1, DER4 and T1, is a member of the interleukin 1 receptor family. The gene of ST2 encodes three splice variants: a soluble secreted form, a transmembrane form and a form of undetermined localization. In some ELISA study on patients with autoimmune diseases, the hST2 concentration of some, but not all, sera of patients with systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), Wegener’s granulomatosis, and Behc¸et disease were markedly higher than those of healthy controls. ST2 protein should be important both to fundamental researches and the further clarification of the clinical implications of the ST2 protein.


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