Human SERPINE1 ELISA Kit0 Publications

カタログ番号: KE00109

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human SERPINE1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant, Cell lysate

アッセイ方法:
Sandwich

感度:
0.16 ng/mL

測定範囲:
0.312-20 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatants 90% 84%-98%
Cell lysates 88% 80%-95%
Human serum 86% 77%-98%
Human plasma 85% 72%-99%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 1.0 0.05 4.8
2 20 4.8 0.06 1.3
3 20 17.6 0.43 2.4

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 1.4 0.04 2.6
2 24 5.2 0.13 2.5
3 24 18.4 0.36 1.9

製品概要
KE00109 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SERPINE1 ELISA kit is to be used to detect and quantify protein levels of endogenous SERPINE1. The assay recognizes human SERPINE1. A polyclonal antibody specific for SERPINE1 has been pre-coated onto the microwells. The SERPINE1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for SERPINE1 is added to detect the captured SERPINE1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
PAI, PAI 1, PAI1, PLANH1, Serpine 1, SERPINE1
背景説明

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. It is produced by the vascular endothelium, the liver, the monocytes/macrophagues, the platelets and the adipose tissue. High plasma levels of PAI-1 have been associated with an increased risk of suffering cardiovascular diseases. It is implicated in the pathogenesis of obesity, insulin resistance and type 2 diabetes. In several tumor types, SERPINE1 expression is up-regulated and it has been described as a poor prognostic marker. Besides its prognostic value, SERPINE1 expression has been validated as a marker for therapy decision making in patients with node-negative breast cancer. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.


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