Human P-selectin ELISA Kit0 Publications

カタログ番号: KE00064

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human P-selectin ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
4.7 pg/mL

測定範囲:
62.5-4000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 95% 84%-113%
Serum 97% 78%-107%
Human plasma 100% 85%-109%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 1,878.5 46.1 2.5
2 20 483.9 14.9 3.1
3 20 118.6 6.2 5.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 20 1,983.3 75.9 3.8
2 20 479.6 18.6 3.9
3 20 104.6 6.4 6.1

製品概要
KE00064 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SELP ELISA kit is to be used to detect and quantify protein levels of endogenous SELP. The assay recognizes human SELP. A polyclonal antibody specific for SELP has been pre-coated onto the microwells. The SELP protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for SELP is added to detect the captured SELP protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selecin, SELP
背景説明

SELP, also named as P-selectin, GMP-140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. Soluble P-selectin is produced by platelets and endothelial cells and circulates in normal plasma.


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