Human S100B ELISA Kit0 Publications

カタログ番号: KE00103

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human S100B ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
1.0 pg/mL

測定範囲:
15.6 - 1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatants 92% 84%-102%
Human plasma 82% 71%-98%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 87.5 3.4 3.9
2 20 302.7 16.2 5.3
3 20 923.9 49.4 5.3

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 90.3 6.5 7.2
2 24 286.2 13.9 4.9
3 24 867.3 46.2 5.3

製品概要
KE00103 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The S100B ELISA kit is to be used to detect and quantify protein levels of endogenous S100B. The assay recognizes human S100B. A polyclonal antibody specific for S100B has been pre-coated onto the microwells. The S100B protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for S100B is added to detect the captured S100B protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
NEF, Protein S100 B, S 100 protein beta chain, S 100 protein subunit beta, S100, S100 calcium binding protein B, S100B, S100beta
背景説明

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. S100B is highly expressed in astrocytes in the brain. It is also expressed in the Schwann cells of the peripheral nervous system, and outside the nervous system in melanocytes, adipocytes and chondrocyte. Elevated S100B levels in cerebrospinal fluid and serum reported after head trauma, subarachnoid hemorrhage, and stroke were correlated with the extent of brain damage. An association between elevated serum S100B and the presence of brain metastases has been reported. Increased S100B levels may also be found in patients with cerebral small vessel disease (SVD).


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