Human MHC class I ELISA Kit

Sensitivity

19.6 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Human

Cat no : KE00035

Synonyms

HLA A, HLA Class I, HLA class I (HLA-A), HLAA, HLA-A, HLA-B, HLA-C, MHC class I antigen A*3



Product Information

KE00035 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MHC classⅠELISA Kit is to be used to detect and quantify protein levels of endogenous MHC classⅠmolecules. The assay recognizes human MHC classⅠmolecules. An MHC classⅠantibody has been pre-coated onto the microwells. The MHC class Ⅰproteins in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for MHC class I is added to detect the captured MHC classⅠproteins. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human MHC class I ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma
Assay type Sandwich
Sensitivity 19.6 pg/mL
Range 31.25-2000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3105

Recovery

Sample TypeAverageRange
Human plasma107%95%-128%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120126.810.18.0
220942.067.27.1
3201,416.3101.37.2

InterAssay

Samplenmean ( pg/mL)SDCV%
124146.615.210.4
2241,085.772.16.6
3241901.1100.85.3

Background Information

Major histocompatibility complex (MHC) antigens, also know in human as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). MHC classⅠmolecules (HLA-A, -B and -C) are polymorphic membrane glycoproteins composed of two polypeptide chains, a heavy alpha chain encoded by variant HLA-A, -B or -C gene and a smaller beta chain (β2-microglobulin). They are peptide-binding proteins that present processed antigens to T lymphocytes. This ELISA kit (KE00035) can be used in the measurement of HLA-A, -B and -C in biological samples.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms HLA A, HLA Class I, HLA class I (HLA-A), HLAA, HLA-A, HLA-B, HLA-C, MHC class I antigen A*3

Publications

SpeciesSample TypeTitle
humancell culture supernatants

J Tissue Eng Regen Med

A bioartificial kidney device with polarized secretion of immune modulators.

Authors - N V Chevtchik
humancell culture supernatants

Sci Rep

Allostimulatory capacity of conditionally immortalized proximal tubule cell lines for bioartificial kidney application.

Authors - Milos Mihajlovic
cell culture supernatants

Tissue Eng Part A

Limited Expansion of Human Hepatocytes in FAH/RAG2-Deficient Swine.

Authors - Erek David Nelson