Human IL23 ELISA Kit0 Publications

カタログ番号: KE00088

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human IL23 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
3.3 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 96% 82%-120%
Human plasma 94% 82%-117%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 775.2 58.7 7.6
2 20 180.3 15.8 8.8
3 20 20.0 1.2 5.7

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 770.1 61.5 8.0
2 24 166.4 15.3 9.2
3 24 13.7 1.2 9.0

製品概要
KE00088 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL23 ELISA kit is to be used to detect and quantify protein levels of endogenous IL23. The assay recognizes human IL23. A monoclonal antibody specific for IL23 has been pre-coated onto the microwells. The IL23 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for IL23 is added to detect the captured IL23 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm and 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
IL 23, IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL-23, IL23A, IL23P19, Interleukin 23 subunit alpha, Interleukin 23 subunit p19, P19, SGRF
背景説明

Interleukin 23 (IL23) is a member of the IL12 cytokine family and composed of two subunits, IL12p40 and IL23p19. It is produced by antigen presenting cells and has been shown to promote the production and survival of a distinct lineage of T-cells called TH17 cells. A functional receptor for IL23 (the IL23 receptor) has been identified and is composed of IL12Rβ1 and IL23R. IL23 is expressed chiefly by the macrophages and DCs. The IL-23R is found on memory T cells, NKT cells, macrophages, DCs, and naive T cells upon activation by TGF-βand IL6. The main biological effects of IL23 identified initially consist of stimulation of antigen presentation by DCs, T cell differentiation to Th17 cells, and production of interferon-γ (IFN-γ). IL23 acts also as an end-stage effector cytokine through direct action on macrophages.


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