Human GMFG ELISA Kit0 Publications

カタログ番号: KE00087

93,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human GMFG ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
13.0 pg/mL

測定範囲:
39-2500 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 90% 78%-104%
Human plasma 96% 76%-128%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 49.6 2.2 4.4
2 20 461.8 8.4 1.8
3 20 1,844.2 70.9 3.9

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 45.6 2.6 5.7
2 24 464.3 17.1 3.7
3 24 1,851.2 29.0 1.6

製品概要
KE00087 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GMFG ELISA kit is to be used to detect and quantify protein levels of endogenous GMFG. The assay recognizes human GMFG. A polyclonal antibody specific for GMFG has been pre-coated onto the microwells. The GMFG protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for GMFG is added to detect the captured GMFG protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Glia maturation factor gamma, glia maturation factor, gamma, GMF GAMMA, GMFG
背景説明

GMFG (glia maturation factor-γ), is a highly conserved brain-specific protein that belongs to the GMF subfamily of the larger Actin-binding protein ADF family. GMFG is preferentially expressed in blood vessel cells and immune cells and its ectopic expression enhances cellular functions such as tube-formation and migration in vitro. GMFG mediates neutrophil and T-lymphocyte migration via regulation of actin cytoskeletal reorganization. GMFG also acts as an intracellular regulator of stress-activated signal transduction by demonstrating activation of p38 MAP kinase and transcription factor NF-kB in astrocytes.


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