Human GDF15 ELISA Kit0 Publications

カタログ番号: KE00108

93,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human GDF15 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant, Urine

アッセイ方法:
Sandwich

感度:
0.3 ng/mL

測定範囲:
1.25-40 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatants 107% 93%-119%
Urine 86% 73%-99%
Human plasma 79% 71%-92%
Human serum 83% 73%-91%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 6.4 0.6 9.4
2 20 15.1 1.6 10.4
3 20 40.6 2.9 7.1

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 6.8 0.4 5.1
2 24 13.9 0.9 6.3
3 24 42.5 1.4 3.3

製品概要
KE00108 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GDF-15 ELISA kit is to be used to detect and quantify protein levels of endogenous GDF-15. The assay recognizes human GDF-15. A polyclonal antibody specific for GDF-15 has been pre-coated onto the microwells. The GDF-15 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for GDF-15 is added to detect the captured GDF-15 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
GDF 15, GDF15, MIC 1, MIC1, NAG 1, NRG 1, NSAID activated gene 1 protein, NSAID regulated gene 1 protein, PDF, PLAB, Placental TGF beta, PTGFB
背景説明

Growth differentiation factor 15 (GDF-15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily. GDF-15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. Its levels are elevated in diseases such as acute respiratory distress syndrome, pulmonary hypertension and heart failure. GDF-15 is associated with cancers, cardiovascular diseases, and inflammatory diseases. GDF-15 may have a paracrine role in the modulation of adipose tissue function and body fat mass. GDF-15 is highly expressed in the placenta. GDF-15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy.


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