Human G-CSF ELISA Kit0 Publications

カタログ番号: KE00106

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

販売および在庫状況はコスモ・バイオ(株)にお問い合わせください。
distributor

Images See all

概要


製品名:
Human G-CSF ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
0.4 pg/mL

測定範囲:
31.25-2000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatants 98% 91%-108%
Human plasma 103% 88%-117%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 433.8 11.6 2.7
2 20 106.5 4.0 4.6
3 20 24.8 4.7 5.0

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 416.7 24.9 6.0
2 24 104.2 5.0 4.8
3 24 24.7 0.9 3.7

製品概要
KE00106 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The G-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous G-CSF. The assay recognizes human G-CSF. A monoclonal antibody specific for G-CSF has been pre-coated onto the microwells. The G-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody specific for G-CSF is added to detect the captured G-CSF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
C17orf33, CSF3, Filgrastim, G CSF, GCSF, G-CSF, Lenograstim, Pluripoietin
背景説明

Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.


Back
to top