Human Fetuin A ELISA Kit0 Publications

カタログ番号: KE00097

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human Fetuin A ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
0.1 ng/mL

測定範囲:
0.313 - 20 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 106% 81%-125%
Human plasma 104% 84%-125%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 1.6 0.05 3.2
2 20 4.6 0.12 2.5
3 20 12.6 0.76 6.1

InterAssay:
Samplenmean (ng/mL)SDCV%
1 20 1.5 0.04 2.8
2 20 4.4 0.23 5.2
3 20 12.9 0.48 3.8

製品概要
KE00097 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Fetuin A ELISA kit is to be used to detect and quantify protein levels of endogenous Fetuin A. The assay recognizes human Fetuin A. A polyclonal antibody specific for Fetuin A has been pre-coated onto the microwells. The Fetuin A protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for Fetuin A is added to detect the captured Fetuin A protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
A2HS, AHS, AHSG, alpha 2 HS glycoprotein, Alpha 2 Z globulin, Ba alpha 2 glycoprotein, FETUA, Fetuin A, HSGA
背景説明

Fetuin A, also named as Alpha2-HS glycoprotein (AHSG), is a member of cystatin superfamily of protease inhibitors. It is a highly expressed glycoprotein in various fetal tissues whereas it is mainly expressed by the liver in adults. Fetuin A, an extracellular inhibitor of transforming growth factor β, is a profibrogenic stimulus in liver disease. Circulating fetuin A may be a beneficial serum biomarker in the detection of liver and vascular fibrosis progression in patients with non-alcoholic fatty liver disease. It is also involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuin A exerts its effects on the cardiovascular system by two different mechanisms. On the one hand it inhibits insulin signaling and induces insulin resistance contributing to the onset of atherosclerosis and on the other hand it inhibits calcium deposition and protects from vascular calcification.


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