Human CD23,FCER2 ELISA Kit0 Publications

カタログ番号: KE00107

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human CD23,FCER2 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant, Urine

アッセイ方法:
Sandwich

感度:
2.2 pg/mL

測定範囲:
31.25-2000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Urine 106% 96%-122%
Human plasma 101% 87%-119%
Cell culture supernatant 104% 82%-117%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 879.9 41.2 4.7
2 20 216.9 9.9 4.6
3 20 49.9 9.7 9.5

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 849.4 72.4 8.5
2 24 209.6 18.5 8.8
3 24 48.2 4.1 8.6

製品概要
KE00107 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD23 ELISA kit is to be used to detect and quantify protein levels of endogenous CD23. The assay recognizes human CD23. A monoclonal antibody specific for CD23 has been pre-coated onto the microwells. The CD23 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody specific for CD23 is added to detect the captured CD23 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor
背景説明

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells. CD23 can be cleaved into soluble fragment (soluble CD23, sCD23) of various sizes displaying pleiotropic biological activities. Increased plasma levels of sCD23 have been reported in allergic disorders, acute viral diseases, lepromatous leprosy, B-CLL, and inflammatory diseases like RA, SLE and sarcoidosis.


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