• Featured Product
  • KD/KO Validated

HDAC2 Polyclonal antibody

HDAC2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 12922-3-AP

Synonyms

HD2, HDAC2, histone deacetylase 2, RPD3, YAF1



Tested Applications

Positive WB detected inHEK-293 cells, human kidney tissue, MCF-7 cells, rat liver tissue, HeLa cells, HepG2 cells, L02 cells, C6 cells, NIH/3T3 cells, rat kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy HDAC2 Ready-To-Use IHC Kit. HDAC2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Immunofluorescence (IF)IF : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

12922-3-AP targets HDAC2 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDAC2 fusion protein Ag3607 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 2
Calculated molecular weight458 aa, 52 kDa; 488 aa,55 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC031055
Gene symbol HDAC2
Gene ID (NCBI) 3066
RRIDAB_2118516
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.

Protocols

Product Specific Protocols
WB protocol for HDAC2 antibody 12922-3-APDownload protocol
IHC protocol for HDAC2 antibody 12922-3-APDownload protocol
IF protocol for HDAC2 antibody 12922-3-APDownload protocol
IP protocol for HDAC2 antibody 12922-3-APDownload protocol
FC protocol for HDAC2 antibody 12922-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Acta Pharm Sin B

Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy.

Authors - Xin Sun
mouseWB

J Clin Invest

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

Authors - Shanshan Zhong
mouseIP

Nat Commun

The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression.

Authors - Xinzhi Li
mouseWB,IP

J Exp Med

ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.

Authors - Longfei Ma
humanWB

Autophagy

Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.

Authors - Qiuhong Shen
  • KD Validated
human,mouseWB,IP

Genome Biol

The histone modification reader ZCWPW1 promotes double-strand break repair by regulating cross-talk of histone modifications and chromatin accessibility at meiotic hotspots

Authors - Shenli Yuan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Zhongwen (Verified Customer) (09-26-2023)

There are two bands close to each other. Maybe they are different isoforms. But the quality is quite good.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
HDAC2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:12922-3-AP)
FH

Iram (Verified Customer) (09-17-2020)

HDAC2 antibody is very good. Giving very clear bands and very good staining in tissue sections

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
  • Cell Tissue Type: Human colon cancer
FH

Dipen (Verified Customer) (09-04-2020)

Excellent antibody. One single clean band at the correct molecular weight.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Neuron
HDAC2 Antibody Western Blot validation (1:1000 dilution) in Neuron (Cat no:12922-3-AP)