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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

ELK1 Polyclonal antibody

ELK1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 27420-1-AP

Synonyms

ELK , ELK1

Validation Data Gallery

Applications:
  • WB analysis using 27420-1-AP

    WB analysis using 27420-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27420-1-AP (ELK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 27420-1-AP

    WB analysis using 27420-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27420-1-AP (ELK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 27420-1-AP

    WB analysis of HepG2 using 27420-1-AP

    WB result of ELK1 antibody (27420-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ELK1 transfected HepG2 cells.

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

27420-1-AP targets ELK1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELK1 fusion protein Ag26683 相同性解析による交差性が予測される生物種
Full Name ELK1, member of ETS oncogene family
Calculated molecular weight 45 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC056150
Gene symbol ELK1
Gene ID (NCBI) 2002
RRIDAB_2880867
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ELK1 antibody 27420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Clin Invest

Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation

Authors - Di Cui
View Article
humanWB

Front Oncol

tRNA-Derived Fragment tRF-Glu-TTC-027 Regulates the Progression of Gastric Carcinoma via MAPK Signaling Pathway

Authors - Weiguo Xu
View Article
ratIHC

J Transl Med

The SETD8/ELK1/bach1 complex regulates hyperglycaemia-mediated EndMT in diabetic nephropathy.

Authors - Xue Li
View Article
humanWB

Biochem Biophys Res Commun

CircRNA circ-0038718 promotes hepatocellular carcinoma progression through sponging miR-139-3p.

Authors - Ranran Sun
View Article
humanWB

Genes Genomics

Tumor-suppressive miRNA-135a inhibits breast cancer cell proliferation by targeting ELK1 and ELK3 oncogenes.

Authors - Akhlaq Ahmad
  • KD Validated
View Article
humanWB

Cancer Res Commun

Adaptive c-Met-PLXDC2 Signaling Axis Mediates Cancer Stem Cell Plasticity to Confer Radioresistance-associated Aggressiveness in Head and Neck Cancer

Authors - Liwei Lang
  • KD Validated
View Article