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DNAJB6 Monoclonal antibody

DNAJB6 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A1D2

Cat no : 66587-1-Ig

Synonyms

DJ4, DnaJ, DNAJB6, Heat shock protein J2, HHDJ1, HSJ 2, HSJ2, MRJ, MSJ 1, MSJ1



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HSC-T6 cells, Neuro-2a cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells
Positive IHC detected inhuman testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66587-1-Ig targets DNAJB6 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen DNAJB6 fusion protein Ag2258 相同性解析による交差性が予測される生物種
Full Name DnaJ (Hsp40) homolog, subfamily B, member 6
Calculated molecular weight 241 aa, 27 kDa
Observed molecular weight 27 kDa, 38 kDa
GenBank accession numberBC000177
Gene symbol DNAJB6
Gene ID (NCBI) 10049
RRIDAB_2881947
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.

Protocols

Product Specific Protocols
WB protocol for DNAJB6 antibody 66587-1-IgDownload protocol
IHC protocol for DNAJB6 antibody 66587-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Transl Oncol

Development of novel DNAJB6-KIAA1522-p-mTOR three-protein prognostic prediction models for CRC

Authors - Yu-Juan Jiang
humanWB

Int J Biol Macromol

DnaJs are enriched in tau regulators

Authors - Abigail R Esquivel
  • KD Validated