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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DMBT1 Polyclonal antibody

DMBT1 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27069-1-AP

Synonyms

DMBT1, Glycoprotein 340, Gp 340, GP340, Hensin, muclin, SAG, Salivary agglutinin, SALSA

Validation Data Gallery

Applications:
  • IHC staining of human stomach cancer using 27069-1-AP

    IHC staining of human stomach cancer using 27069-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27069-1-AP (DMBT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 27069-1-AP

    IHC staining of human stomach cancer using 27069-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27069-1-AP (DMBT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 27069-1-AP

    IHC staining of human kidney using 27069-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27069-1-AP (DMBT1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 27069-1-AP

    IHC staining of human kidney using 27069-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27069-1-AP (DMBT1 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of human brain using 27069-1-AP

    IF Staining of human brain using 27069-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human brain tissue using DMBT1 antibody (27069-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human brain using 27069-1-AP

    IF Staining of human brain using 27069-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human brain tissue using DMBT1 antibody (27069-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman stomach cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman brain tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27069-1-AP is DMBT1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DMBT1 fusion protein Ag16229 相同性解析による交差性が予測される生物種
Full Name deleted in malignant brain tumors 1
Calculated molecular weight 2413 aa, 261 kDa
GenBank accession numberBC153299
Gene symbol DMBT1
Gene ID (NCBI) 1755
RRIDAB_2880741
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Deleted in malignant brain tumors 1 (DMBT1), also known as the salivary scavenger and agglutinin (SALSA), salivary agglutinin (SAG) or gp340, is a multifunctional molecule with important functions in innate immunity, inflammation and epithelial homeostasis (PMID: 28668353). DMBT1 has some isoforms with the molecular weight of 193, 260 and 340 kDa.

Protocols

Product Specific Protocols
IHC protocol for DMBT1 antibody 27069-1-APDownload protocol
IF protocol for DMBT1 antibody 27069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols