DEFA6 Polyclonal antibody

DEFA6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17923-1-AP

Synonyms

DEF6, DEFA6, Defensin 6, Defensin, alpha 6, defensin, alpha 6, Paneth cell, HD 6



Tested Applications

Positive WB detected inCOLO 320 cells, mouse small intestine tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DEFA6 Ready-To-Use IHC Kit. DEFA6 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

17923-1-AP targets DEFA6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited ReactivityHuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DEFA6 fusion protein Ag12341 相同性解析による交差性が予測される生物種
Full Name defensin, alpha 6, Paneth cell-specific
Calculated molecular weight 100 aa, 11 kDa
GenBank accession numberBC093951
Gene symbol DEFA6
Gene ID (NCBI) 1671
RRIDAB_2878468
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. DEFA6 is alpha 6 of Defensin. This protein is secret expression and can be cleaved in to the fragments with MW 4-6 kDa. It can be used as a maker of Paneth cell. DEFA6 has very low antimicrobial activity against Gram-negative and Gram-positive bacteria. it may protect cells against infection with HIV-1.

Protocols

Product Specific Protocols
WB protocol for DEFA6 antibody 17923-1-APDownload protocol
IHC protocol for DEFA6 antibody 17923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
HumanIF

J Clin Invest

Human intestinal bitter taste receptors regulate innate immune responses and metabolic regulators in obesity.

Authors - Kathrin I Liszt