CCDC8 Polyclonal antibody

CCDC8 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 27194-1-AP

Synonyms

CCDC8



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27194-1-AP is CCDC8 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC8 fusion protein Ag25794 相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 8
Observed molecular weight 90 kDa, 59 kDa
GenBank accession numberBC025243
Gene symbol CCDC8
Gene ID (NCBI) 83987
RRIDAB_2880795
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Coiled-coil domain-containing protein 8 (CCDC8) is a 538-amino acid protein with the molecular mass of 59kD and 90kD cause of the posttranslational modifications including amidation, glycosylation, phosphorylation, and myristalation (PMID: 21737058). CCDC8 has 2 coiled-coil domains, and the proteins containing this domain are involved in diverse biological processes, such as the regulation of gene expression, cell division, and membrane fusion. CCDC8 is evolutionarily conserved, and CCDC8 mutation leads to the development of 3M syndrome in humans, a primordial growth disorder, by interacting with CUL7, OBSL1, and P53. Low-level or no expression of CCDC8 was shown to be closely related to the development of some tumors, such as renal cell carcinoma, multiple myeloma, breast cancer, and prostate cancer (PMID: 21737058, 27342910).

Protocols

Product Specific Protocols
WB protocol for CCDC8 antibody 27194-1-APDownload protocol
IHC protocol for CCDC8 antibody 27194-1-APDownload protocol
IP protocol for CCDC8 antibody 27194-1-APDownload protocol
Standard Protocols
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