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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C4-B Alpha Chain/C4b-B/C4d-B Polyclonal antibody

C4-B Alpha Chain/C4b-B/C4d-B Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 55228-1-AP

Synonyms

C4, C4B, C4B1, C4B12, C4B2, C4B3, C4B5, C4F, CH, CO4, Complement C4-B, CPAMD3

Validation Data Gallery

Applications:
  • WB analysis of human blood using 55228-1-AP

    WB analysis of human blood using 55228-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 55228-1-AP (C4-B alpha chain/C4b-B/C4d-B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman blood

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

55228-1-AP targets C4-B Alpha Chain/C4b-B/C4d-B in WB,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name complement component 4B (Chido blood group)
Calculated molecular weight 193 kDa
Observed molecular weight 95 kDa, 300 kDa
GenBank accession numberNM_000592
Gene symbol C4B
Gene ID (NCBI) 721
RRIDAB_2881291
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1116-1128aa of human C4-B protein can recognize C4-B precursor, C4-B alpha chain, C4b-B, and C4d-B.

Protocols

Product Specific Protocols
WB protocol for C4-B Alpha Chain/C4b-B/C4d-B antibody 55228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols