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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ARNT,HIF1B Monoclonal antibody

ARNT,HIF1B Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3B12C5

Cat no : 66732-1-Ig

Synonyms

ARNT, ARNT protein, ARNT,HIF1B, bHLHe2, HIF 1 beta, HIF 1beta, HIF1 beta, HIF1B, HIF1BETA, TANGO

Validation Data Gallery

Applications:
  • WB analysis using 66732-1-Ig

    WB analysis using 66732-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 66732-1-Ig

    WB analysis of NIH/3T3 using 66732-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66732-1-Ig

    WB analysis using 66732-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66732-1-Ig

    IF Staining of HepG2 using 66732-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66732-1-Ig (ARNT,HIF1B antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 66732-1-Ig

    FC experiment of HeLa using 66732-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ARNT,HIF1B (66732-1-Ig, Clone:3B12C5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of HeLa using 66732-1-Ig

    FC experiment of HeLa using 66732-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ARNT,HIF1B (66732-1-Ig, Clone:3B12C5) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inCaco-2 cells, HeLa cells, NIH/3T3 cells, HepG2 cells, HT-29 cells, HSC-T6 cells
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ARNT Ready-To-Use IHC Kit. ARNT primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

66732-1-Ig targets ARNT,HIF1B in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ARNT,HIF1B fusion protein Ag5507 相同性解析による交差性が予測される生物種
Full Name aryl hydrocarbon receptor nuclear translocator
Calculated molecular weight 416 aa, 46 kDa, 87 kDa
Observed molecular weight 87 kDa
GenBank accession numberBC060838
Gene symbol ARNT
Gene ID (NCBI) 405
RRIDAB_2882082
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HIF1B, also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia

Protocols

Product Specific Protocols
WB protocol for ARNT,HIF1B antibody 66732-1-IgDownload protocol
IF protocol for ARNT,HIF1B antibody 66732-1-IgDownload protocol
FC protocol for ARNT,HIF1B antibody 66732-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols