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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADRM1 Monoclonal antibody

ADRM1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A3

Cat no : 66464-1-Ig

Synonyms

adhesion regulating molecule 1, ADRM1, ARM 1, ARM1, GP110, hRpn13, Rpn13, Rpn13 homolog

Validation Data Gallery

Applications:
  • WB analysis using 66464-1-Ig

    WB analysis using 66464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66464-1-Ig (ADRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66464-1-Ig

    IF Staining of HeLa using 66464-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ADRM1 antibody (66464-1-Ig, Clone: 1A1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Raji cells
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ADRM1 Ready-To-Use IHC Kit. ADRM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66464-1-Ig targets ADRM1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen ADRM1 fusion protein Ag24699 相同性解析による交差性が予測される生物種
Full Name adhesion regulating molecule 1
Calculated molecular weight 407 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC017245
Gene symbol ADRM1
Gene ID (NCBI) 11047
RRIDAB_2881832
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.

Protocols

Product Specific Protocols
WB protocol for ADRM1 antibody 66464-1-IgDownload protocol
IF protocol for ADRM1 antibody 66464-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols