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ACSL4/FACL4 Monoclonal antibody

ACSL4 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1H5D3

Cat no : 66617-1-Ig

Synonyms

ACS4, ACSL4, FACL4, LACS 4, LACS4, MRX63, MRX68



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Caco-2 cells, COLO 320 cells, L02 cells, HT-29 cells
Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy ACSL4 Ready-To-Use IHC Kit. ACSL4 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 11 publications below

Product Information

66617-1-Ig targets ACSL4 in WB, IHC, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACSL4 fusion protein Ag18085 相同性解析による交差性が予測される生物種
Full Name acyl-CoA synthetase long-chain family member 4
Calculated molecular weight 711 aa, 79 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC034959
Gene symbol ACSL4
Gene ID (NCBI) 2182
RRIDAB_2881977
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACSL4 antibody 66617-1-IgDownload protocol
IHC protocol for ACSL4 antibody 66617-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Free Radic Biol Med

Targeting the Na+/K+ ATPase DR-region with DR-Ab improves doxorubicin-induced cardiotoxicity

Authors - Bin Leng
humanWB

J Med Chem

Seco-Lupane Triterpene Derivatives Induce Ferroptosis through GPX4/ACSL4 Axis and Target Cyclin D1 to Block the Cell Cycle.

Authors - Haohao Wang
humanWB

Front Pharmacol

Targeting Mitochondrial ROS-Mediated Ferroptosis by Quercetin Alleviates High-Fat Diet-Induced Hepatic Lipotoxicity.

Authors - Jin-Jin Jiang
humanWB

Ann Transl Med

Atractylodin may induce ferroptosis of human hepatocellular carcinoma cells.

Authors - Yongfei He
ratWB

Cell Stress Chaperones

Activation of NRF2/FPN1 pathway attenuates myocardial ischemia-reperfusion injury in diabetic rats by regulating iron homeostasis and ferroptosis.

Authors - Hao Tian
WB

FEBS Open Bio

Lipoxin A4 protects primary spinal cord neurons from Erastin-induced ferroptosis by activating the Akt/Nrf2/HO-1 signaling pathway.

Authors - Na Wei