ACOT9 Monoclonal antibody
ACOT9 Monoclonal Antibody for IHC, WB,ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Rat, mouse and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3F10G5
Cat no : 66532-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HEK-293 cells, RAW 264.7 cells, Jurkat cells, HSC-T6 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ACOT9 Ready-To-Use IHC Kit. ACOT9 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
Product Information
66532-1-Ig targets ACOT9 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, mouse samples.
| Tested Reactivity | Human, Rat, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ACOT9 fusion protein Ag8884 相同性解析による交差性が予測される生物種 |
| Full Name | acyl-CoA thioesterase 9 |
| Calculated molecular weight | 24 kDa, 49 kDa |
| Observed molecular weight | 43-50 kDa |
| GenBank accession number | BC012573 |
| Gene symbol | ACOT9 |
| Gene ID (NCBI) | 23597 |
| RRID | AB_2881895 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACOT9 (Acyl-coenzyme A thioesterase 9) is a member of the acyl-CoA thioesterase superfamily, which is a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. ACOT9 has 4 isoforms with the molecular mass of 43-50 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACOT9 antibody 66532-1-Ig | Download protocol |
| IHC protocol for ACOT9 antibody 66532-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma |
