mCherry Polyclonal antibody
mCherry Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein and More (6)
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 26765-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Transfected HEK-293 cells |
Positive IF detected in | Transfected HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 37 publications below |
IHC | See 2 publications below |
IF | See 21 publications below |
IP | See 6 publications below |
CoIP | See 3 publications below |
Product Information
The immunogen of 26765-1-AP is mCherry Fusion Protein expressed in E. coli.
Tested Reactivity | recombinant protein |
Cited Reactivity | human, D. punctatus, yeast, mouse, zebrafish, plant |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | mCherry fusion protein Ag25320 相同性解析による交差性が予測される生物種 |
Full Name | mCherry |
Calculated molecular weight | 27 kDa |
Gene symbol | |
Gene ID (NCBI) | |
RRID | AB_2876881 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively.
Protocols
Product Specific Protocols | |
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WB protocol for mCherry antibody 26765-1-AP | Download protocol |
IF protocol for mCherry antibody 26765-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Extracell Vesicles Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion | ||
Protein Cell cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence. | ||
Dev Cell Centrosomal Localization of RXRα Promotes PLK1 Activation and Mitotic Progression and Constitutes a Tumor Vulnerability. | ||
Autophagy Proteotoxic stress disrupts epithelial integrity by inducing MTOR sequestration and autophagy overactivation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Verdiana (Verified Customer) (10-12-2023) | Nice and clear bands!
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FH Andrea (Verified Customer) (10-05-2023) | Good and strong signal for the mCherry fusion protein. In my case it was a 1:1000 dilution.
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FH Tatyana (Verified Customer) (01-09-2023) | Excellent antibody, very good bright signal, no background or non-specific bands. HeLa cells were transfected with plasmids for expression of mCherry tagged proteins, lysed after 24 hours, followed by WB (semi-dry transfer, blocking in 4% milk). Antibody was diluted in 4% milk.
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FH Laura (Verified Customer) (01-14-2020) | Antibody was used in 5% TBST overnight, gave a clean blot.
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FH Benjamin (Verified Customer) (12-10-2019) | This antibody has been used to image mCherry fusion proteins expressed in HEK293T cells via western blot with high rates of success.Antibody was diluted in 3% milk TBST and incubated overnight at 4 degrees.
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FH Liang (Verified Customer) (05-27-2019) | The antibody works perfect. According the attached image, The 2nd from the left is the negative control protein (mGFP) and the 3rd lane is the mCherry protein as the positive control, the others are the target protein.
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