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USP1 Monoclonal antibody
USP1 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1C10H6
Cat no : 66069-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HeLa cells |
Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
66069-1-Ig targets USP1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
Tested Reactivity | human, rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | USP1 fusion protein Ag5904 相同性解析による交差性が予測される生物種 |
Full Name | ubiquitin specific peptidase 1 |
Calculated molecular weight | 88 kDa |
Observed molecular weight | 100 kDa |
GenBank accession number | BC050525 |
Gene symbol | USP1 |
Gene ID (NCBI) | 7398 |
RRID | AB_11043340 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2.It is Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA.This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases(PMID:9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment(PMID:18082604).
Protocols
Product Specific Protocols | |
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WB protocol for USP1 antibody 66069-1-Ig | Download protocol |
IHC protocol for USP1 antibody 66069-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |