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  • KD/KO Validated

SQRDL Polyclonal antibody

SQRDL Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17256-1-AP

Synonyms

CGI 44, SQOR, SQRDL



Tested Applications

Positive WB detected inHeLa cells, HT-29 cells, HepG2 cells, THP-1 cells, HepG cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, human testis tissue, human colon cancer tissue, human liver cancer tissue, mouse liver tissue, rat liver tissue, human spleen tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SQRDL Ready-To-Use IHC Kit. SQRDL primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17256-1-AP targets SQRDL in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, chicken, rat, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SQRDL fusion protein Ag10762 相同性解析による交差性が予測される生物種
Full Name sulfide quinone reductase-like (yeast)
Calculated molecular weight 450 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC016836
Gene symbol SQRDL
Gene ID (NCBI) 58472
RRIDAB_2195894
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SQRDL(Sulfide:quinone oxidoreductase, mitochondrial) catalyzes the oxidation of hydrogen sulfide, with the help of a quinone.Although the SQRDL precursor protein displays a molecular weight of 50 kDa,a prominent band at about 46 kDa is detected by western blot. This discrepancy reflects the cleavage of an N-terminal targeting sequence of 4 kDa during the import of the protein into the mitochondria(PMID:22067608).SQRDL is one of the enzymes involved in H2S signaling in a discrete population of neurons, oligodendrocytes, and endothelial cells.

Protocols

Product Specific Protocols
WB protocol for SQRDL antibody 17256-1-APDownload protocol
IHC protocol for SQRDL antibody 17256-1-APDownload protocol
IP protocol for SQRDL antibody 17256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell

Endogenous Hydrogen Sulfide Production Is Essential for Dietary Restriction Benefits.

Authors - Christopher Hine
  • KD Validated
mouse,ratWB

Nat Commun

Sulfide catabolism ameliorates hypoxic brain injury.

Authors - Eizo Marutani
  • KD Validated
mouseWB

EMBO Mol Med

β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.

Authors - Agustín Hidalgo-Gutiérrez
humanWB

EMBO Mol Med

Coenzyme Q deficiency causes impairment of the sulfide oxidation pathway.

Authors - Marcello Ziosi
mouseWB

EMBO Mol Med

CoQ deficiency causes disruption of mitochondrial sulfide oxidation, a new pathomechanism associated with this syndrome.

Authors - Marta Luna-Sánchez
mouseWB

Antioxid Redox Signal

The Cysteine Dioxgenase Knockout Mouse: Altered Cysteine Metabolism in Nonhepatic Tissues Leads to Excess H2S/HS(-) Production and Evidence of Pancreatic and Lung Toxicity.

Authors - Roman Heather B HB