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Species Reactivity

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  • Featured Product
  • KD/KO Validated

SPRED2 Polyclonal antibody

SPRED2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24091-1-AP

Synonyms

Spred 2, SPRED2

Validation Data Gallery

Applications:
  • WB analysis of MCF-7 using 24091-1-AP

    WB analysis of MCF-7 using 24091-1-AP

    WB result of SPRED2 antibody (24091-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPRED2 transfected MCF-7 cells.

  • WB analysis using 24091-1-AP

    WB analysis using 24091-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24091-1-AP

    WB analysis using 24091-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-12 using 24091-1-AP

    WB analysis of PC-12 using 24091-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 24091-1-AP

    WB analysis of mouse testis using 24091-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 24091-1-AP

    WB analysis of mouse brain using 24091-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 24091-1-AP

    WB analysis of rat brain using 24091-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 24091-1-AP (SPRED2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 24091-1-AP

    IHC staining of rat testis using 24091-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 24091-1-AP (SPRED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 24091-1-AP

    IHC staining of rat testis using 24091-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 24091-1-AP (SPRED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 24091-1-AP

    IHC staining of human testis using 24091-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24091-1-AP (SPRED2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 24091-1-AP

    IHC staining of human testis using 24091-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24091-1-AP (SPRED2 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 24091-1-AP

    IF Staining of HeLa using 24091-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24091-1-AP (SPRED2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-12 cells, MCF-7 cells, mouse testis tissue, mouse brain tissue, rat brain tissue, T-47D cells, rat testis tissue
Positive IHC detected inrat testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

24091-1-AP targets SPRED2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPRED2 fusion protein Ag21359 相同性解析による交差性が予測される生物種
Full Name sprouty-related, EVH1 domain containing 2
Calculated molecular weight 418 aa, 48 kDa
Observed molecular weight 40-48 kDa
GenBank accession numberBC136334
Gene symbol SPRED2
Gene ID (NCBI) 200734
RRIDAB_2879429
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPRED2 is a member of the SPRED family of proteins that modulate GFR signaling by inhibiting the Ras-MAPK cascade (PMID: 17094949). SPRED family members are characterized by an N-terminal Enabled/VASP homology 1 domain (EVH1), a central c-Kit binding domain (KBD) and a C-terminal Sprouty-related domain (SPR) (PMID: 17691106). Three mammalian SPREDs (SPRED1-3) have been described. In adult tissues, SPRED2 is expressed ubiquitously, but most highly expressed in glandular epithelia (PMID: 15580519; 17691106). Reduced expression level of SPRED2 has been reported in human hepatocellular carcinoma (HCC), suggesting SPRED2 may play a role in tumorgenesis (PMID: 16652141). Gene disruption of SPRED2 causes dwarfism (PMID: 15946934).

Protocols

Product Specific Protocols
WB protocol for SPRED2 antibody 24091-1-APDownload protocol
IHC protocol for SPRED2 antibody 24091-1-APDownload protocol
IF protocol for SPRED2 antibody 24091-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

PLoS One

Expression of Spred2 in the urothelial tumorigenesis of the urinary bladder

Authors - Shinsuke Oda
View Article
mouseWB

Biol Pharm Bull

Inhibition of Spred/Sprouty Expression in the Skin of a Contact Dermatitis-Like Model.

Authors - Hiroyasu Sakai
View Article
mouseIF

Histol Histopathol

microRNA-141-3p mediates epithelial cell proliferation, apoptosis, and epithelial-mesenchymal transition and alleviates pulmonary fibrosis in mice via Spred2

Authors - Liang Zhu
View Article
humanWB

Int J Mol Sci

SPRED2: A Novel Regulator of Epithelial-Mesenchymal Transition and Stemness in Hepatocellular Carcinoma Cells

Authors - Tong Gao
View Article