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SMCR7/MID49 Polyclonal antibody
SMCR7/MID49 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16413-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HepG2 cells, HT-1080 cells |
| Positive IHC detected in | human liver cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 10 publications below |
| WB | See 50 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
Product Information
16413-1-AP targets SMCR7/MID49 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SMCR7/MID49 fusion protein Ag9531 相同性解析による交差性が予測される生物種 |
| Full Name | Smith-Magenis syndrome chromosome region, candidate 7 |
| Calculated molecular weight | 454 aa, 49 kDa |
| Observed molecular weight | 49 kDa |
| GenBank accession number | BC014973 |
| Gene symbol | SMCR7 |
| Gene ID (NCBI) | 125170 |
| RRID | AB_2714217 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SMCR7, also named as MID49, is a 49 kDa mitochondrial outer membrane protein which regulates mitochondrial morphology. SMCR7 can directly recruit the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. SMCR7 has two isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SMCR7/MID49 antibody 16413-1-AP | Download protocol |
| IHC protocol for SMCR7/MID49 antibody 16413-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division. | ||
Cell Metab Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells. | ||
Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
| ||
Autophagy Loss of MIEF1/MiD51 confers susceptibility to BAX-mediated cell death and PINK1-PRKN-dependent mitophagy.
| ||
J Cell Biol The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission. | ||
EMBO J FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Chun (Verified Customer) (07-03-2019) | This antibody is very good.
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