• Featured Product
  • KD/KO Validated

MCT4 Polyclonal antibody

MCT4 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 22787-1-AP

Synonyms

MCT 4, MCT4, Monocarboxylate transporter 3, Monocarboxylate transporter 4, SLC16A3



Tested Applications

Positive WB detected inHeLa cells, rat skeletal muscle tissue, HepG2 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human renal cell carcinoma tissue, mouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MCT4 Ready-To-Use IHC Kit. MCT4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

22787-1-AP targets MCT4 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity human, goat, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCT4 fusion protein Ag18788 相同性解析による交差性が予測される生物種
Full Name solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
Calculated molecular weight 465 aa, 49 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC112269
Gene symbol SLC16A3
Gene ID (NCBI) 9123
RRIDAB_11182479
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The monocarboxylate transporter 4 (MCT4, also known as SLC16A3) is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate and ketone bodies. It is widely expressed, particularly strongly in glycolytic tissues such as white skeletal muscle fibres, astrocytes, white blood cells, chondrocytes and some mammalian cell lines. MCT4 is also linked to tumor biology because it mediates lactate transport across membranes resulting in antiapoptotic effects.

Protocols

Product Specific Protocols
WB protocol for MCT4 antibody 22787-1-APDownload protocol
IHC protocol for MCT4 antibody 22787-1-APDownload protocol
IF protocol for MCT4 antibody 22787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Cancer

Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer

Authors - Federica Cappellesso
humanWB

Nat Metab

Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma

Authors - Zijian Yang
  • KD Validated

Adv Sci (Weinh)

SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle

Authors - Xiaowei She
mouseIF

Nat Commun

Lactate sensing mechanisms in arterial chemoreceptor cells.

Authors - Hortensia Torres-Torrelo
mouseWB

Adv Healthc Mater

Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature.

Authors - Zhijie Jiang
humanWB

Proc Natl Acad Sci U S A

Monocarboxylate transporter antagonism reveals metabolic vulnerabilities of viral-driven lymphomas.

Authors - Emmanuela N Bonglack

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Aderonke (Verified Customer) (05-26-2023)

Immunoblotting was done to show the expression of MCT 4 in five head and neck cancer cell lines; only three of the five cell lines demonstrated this. An inhibitor was thereafter used at two differenct concentrations in UM-SCC-17A, and the expression decreased with increasing concentrations.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:20,000
  • Cell Tissue Type: Head and neck cancer cell line
FH

Juan (Verified Customer) (07-15-2019)

We used the antibody for WB method. The band backgound is clear.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Hela cells