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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEC61B Polyclonal antibody

SEC61B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15087-1-AP

Synonyms

Sec61 beta subunit, SEC61B

Validation Data Gallery

Applications:
  • WB analysis using 15087-1-AP

    WB analysis using 15087-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15087-1-AP (SEC61B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15087-1-AP

    WB analysis of HeLa using 15087-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15087-1-AP (SEC61B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 15087-1-AP

    WB analysis of HepG2 using 15087-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15087-1-AP (SEC61B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 15087-1-AP

    IP experiment of HepG2 using 15087-1-AP

    IP result of anti-SEC61B (IP:15087-1-AP, 4ug; Detection:15087-1-AP 1:500) with HepG2 cells lysate 1800ug.

  • IHC staining of human liver using 15087-1-AP

    IHC staining of human liver using 15087-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15087-1-AP (SEC61B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 15087-1-AP

    IHC staining of human liver using 15087-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15087-1-AP (SEC61B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 15087-1-AP

    IHC staining of human cervical cancer using 15087-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15087-1-AP (SEC61B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 15087-1-AP

    IHC staining of human cervical cancer using 15087-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15087-1-AP (SEC61B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse pancreas using 15087-1-AP

    IF Staining of mouse pancreas using 15087-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (15087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse pancreas using 15087-1-AP

    IF Staining of mouse pancreas using 15087-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (15087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 15087-1-AP

    IF Staining of HepG2 using 15087-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SEC61B antibody (15087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 15087-1-AP

    IF Staining of HeLa using 15087-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15087-1-AP (SEC61B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells, mouse pancreas tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15087-1-AP targets SEC61B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC61B fusion protein Ag7169 相同性解析による交差性が予測される生物種
Full Name Sec61 beta subunit
Calculated molecular weight 10 kDa
Observed molecular weight 10-15 kDa
GenBank accession numberBC001734
Gene symbol SEC61B
Gene ID (NCBI) 10952
RRIDAB_2186411
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).

Protocols

Product Specific Protocols
WB protocol for SEC61B antibody 15087-1-APDownload protocol
IHC protocol for SEC61B antibody 15087-1-APDownload protocol
IF protocol for SEC61B antibody 15087-1-APDownload protocol
IP protocol for SEC61B antibody 15087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

QRICH1 dictates the outcome of ER stress through transcriptional control of proteostasis.

Authors - Kwontae You
View Article
mouseWB

Nat Neurosci

Faulty autolysosome acidification in Alzheimer's disease mouse models induces autophagic build-up of Aβ in neurons, yielding senile plaques.

Authors - Ju-Hyun Lee
View Article
humanWB

Sci Adv

CReP mediates selective translation initiation at the endoplasmic reticulum.

Authors - Jonathan P Kastan
View Article
humanIF

J Cell Biol

Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway.

Authors - Satoshi Ninagawa
View Article
humanIF

Genes Dev

Identification of a localized nonsense-mediated decay pathway at the endoplasmic reticulum.

Authors - Dasa Longman
View Article
human,mouseWB

Mol Ther

Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation.

Authors - Xinjie Wang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Wei (Verified Customer) (09-22-2020)

SEC61B Antibody have correct localization on ER.

  • Applications: Immunofluorescence
  • Cell Tissue Type: Mouse heart
FH

WEI (Verified Customer) (06-29-2020)

Strong band for heart tissues, but not so sharp band.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse heart